These data comprise remotely sensed column concentrations of greenhouse gases over Jinja, Uganda, covering the period spanning January to April 2020. A Bruker EM27/SUN spectrometer and solar tracker were used to make the measurements, which were then processed into column concentrations of carbon dioxide, methane, and carbon monoxide using the PROFFAST retrieval code developed through the COllaborative Carbon Column Observing Network (COCCON) programme at the Karlsruhe Institute of Technology. The data were collected to provide a source of validation for satellite data products, and for outputs from atmospheric chemistry and transport models. The data were collected and processed by Neil Humpage at the University of Leicester, in collaboration with William Okello at the National Fisheries Resources Research Institute who provided the measurement site.